The Phadia 250 is a fully automated, assay processing instrument used for in vitro testing in clinical areas such as allergy, asthma, and autoimmunity. The Phadia 250 uses ImmunoCAP solid phase technology and can extract the analyte molecules from the sample since it has a three-dimensional structure covered with a large amount of either antigens/allergens (binding specific antibody analytes) or specific antibodies (binding antigen analytes). These antigens/allergens or antibodies are covalently coupled to the solid phase and react with the molecule of interest in the patient sample. The amplifier molecule has the function to specifically bind to the analyte molecule of interest (using an antibody against the analyte) and to carry an enzyme able to perform an enzyme reaction. In the ImmunoCAP system a non-fluorescent substrate is used. During the enzyme reaction, the substrate is converted to a fluorescent product. The fluorescence is directly proportional to the concentration of the analyte.

Manufacturer: ThermoFisher Scientific
Serial #: N02671
Year installed: N/A